Increased plasma adiponectin levels have been reported in type 1 diabetic patients, however studies. The mechanisms of alloxan and streptozotocininduced diabetes. Streptozotocin, type i diabetes severity and bone katherine motyl and laura r. Diabetesinduced desensitization to ndmrs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of ndmrs. Impact of streptozotocin induced diabetes and insulin therapy on plasma adiponectin levels adiponectin also known as acr adiponectin also known as acrp 30, adipoq, and gbp28 is an adipokine implicated in the regulation of insulin sensitivity. The blood glucose levels reached the maximum after 30 minutes of administration of 2 g glucosekg bw and then reduced to initial levels within 2 hours in all groups except diabetic rats that showed severe glucose intolerance throughout the experimental. Streptozotocin stzinduced diabetes mellitus dm offers a very costeffective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. Changes in oxidative stress and antioxidant enzyme activities.
In a study with male icr mice, the effects of ursolic acid 0. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Diabetes was induced in rats by single intraperitoneal injection of streptozotocin 45mgkg body weight. Impact of streptozotocininduced diabetes and insulin therapy on plasma adiponectin levels adiponectin also known as acr adiponectin also known as acrp 30, adipoq, and gbp28 is an adipokine implicated in the regulation of insulin sensitivity. Furthermore explore the role of age in cpeptide and nitric oxide no and their relation in stz induced diabetic rats. Original article effects of panax quinquefolium on. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Fvbnj 001800, balbcj 000651, and aj 000646 males are resistant. This project has assessed cardiovascular, ocular and neuropathic changes over a. Diabetes resulted in impairment of growth rate and excretion of glucose in the urine. Key words streptozotocin, glucose levels, induced diabetic, bodyweight. Pdf induction of diabetes by streptozotocin in rats.
Evaluation of antidiabetic activity of ethanolic extract of. Streptozotocin use in mice and rats version 1 date of publication. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by the glucose tolerance test. Stz induced diabetes are commonly performed in male wistar kyoto rats. Unlike type 1 diabetes, type 2 diabetes t2d is associated with resistance to insulin action. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Multiple low dose streptozotocininduced diabetes in the rat. Hyperglycemia enhances the production of reactive oxygen species. The most common animal model of human diabetes is streptozotocin stz induced diabetes in the rat.
In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. Comparative antidiabetic activity of methanolic extract of. Diabetes is induced in mice by using streptozotocin stz, a compound that has a preferential toxicity toward pancreatic. Streptozotocininduced diabetic models in mice and rats. Therapeutic development to prevent bone loss andor restore lost bone in t1 diabetic patients requires. At 99g body weight, diabetes was induced in some of the rats by intravenous administration of streptozotocin a gift of upjohn ltd. Streptozotocininduced type 1 diabetes in rodents as a model. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland. As many as 50% of adults with type i t1 diabetes exhibit bone loss and are at increased risk for fractures. Streptozotocinhigh fat dietinduced type 2 diabetes. The streptozotocin induced diabetic rats showed significant reductions in biochemical parameter after treatment with the extract and glibenclamide used as standard as compared to the diabetic controls.
The ethanolic extract of alternanthera sessilis linn. The responses are accompanied by corresponding inverse changes in. Therefore, four groups of rats were allocated as control c, stzinduced dm d, and. Three days after degeneration of beta cells, diabetes was induced in all animals. The booklet describes gestational diabetes, its causes, and its features and. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. Effect of streptozotocin on glucose levels in albino wister rats. Jan 11, 2018 the epidemic of type 2 diabetes mellitus t2dm is fueled by added fructose consumption. Hypoglycemia low blood glucose diabetes education services. In conclusion, the streptozotocininduced diabetes mellitus in rats was stable for 6 months, afterward, however, a significant recovery developed in. Diabetes mellitus is a metabolic disease, which progresses with chronic hyperglycaemia related to absolute and relative insulin deficiency, and it is also induced by genetic or environmental factors.
Streptozotocin stz is an antibiotic that produces pancreatic islet. Recognizing the signs of high and low blood sugar is crucial. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days, 60mgkg of streptozotocin was injected intravenously. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and house in a 12h light, 12h dark cycle environment, temperature 25c, with standard rat diet and water in metabolic cages for one week prior to. Streptozotocin is a glucosaminenitrosourea compound. In streptozotocin and highfat diet induced diabetes model of mice, it is shown that ursolic acid protected pancreatic.
Diabetes handouts for providers and educators scripps health. We evaluated nude male mice from various sources for their sensitivity to a single high dose 160 to 240 mgkg of stz. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. It means your blood glucose levels are higher than normal but arent high enough to be called diabetes. Streptozotocininduced experimental diabetes in male wistar rats. Streptozotocin stz is an alkylating agent that affects pancreatic islets, inducing diabetes in mice. Full text streptozotocininduced type 1 diabetes in rodents. The resistance to ndmrs was stronger in the later stage of diabetes 16 versus 4 weeks after stz treatment. Streptozotocinhigh fat dietinduced type 2 diabetes bienta.
Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. The streptozotocin produced a stable form of diabetes, with blood changes consistent with the disease, and only minor. Detailed in this unit are protocols for producing stzinduced insulin deficiency and hyperglycemia in mice and rats. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by. Induction of diabetes mellitus in rats using intraperitoneal. Diabetes induced desensitization to ndmrs depended on the stage of diabetes and on the different kind of muscles observed while was independent on different kind of ndmrs. Longterm studies on pathological changes related to hyperglycaemia require a. Streptozotocininduced type 1 diabetes in rodents as a. Introduction diabetes mellitus, often simply referred to as diabetes.
The epidemic of type 2 diabetes mellitus t2dm is fueled by added fructose consumption. Mrinmay das2, ashok kumar d1, mastanaiah k2 and arup das2. Streptozotocininduced diabetic ratsmice stz model advantages. Evaluation of antidiabetic activity of ethanolic extract of alternanthera sessilis linn. Changes in oxidative stress and antioxidant enzyme. Multiple low dose streptozotocin induced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. To develop the corresponding animal pathology model, high fat diet hfd is often used. Three days after stz induction, the diabetes rats were treated with aqueous extract of erythrina variegata l. Alhagi maurorum camel thorn plant is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. Cdcs division of diabetes translation offers many fact sheets about diabetes.
Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Thirty white male sprague dawley rats weighing 150200 gm, about 4 month old were equally divided into the following. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. Animals models for studying diabetes mellitus etuk, e. After induction of diabetes, consumption of food and water and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body decreased in the diabetic animals. The mmpc is a national institutes of healthsponsored resource that provides experimental testing services to scientists studying diabetes, obesity, diabetic. Impact of streptozotocininduced diabetes and insulin therapy.
The mechanism of alloxan and streptozotocin action in b cells. What it means for you english, low literacy pdf very low literacy pdf. Some reports suggest cellular toxicity outside of the pancreas. Diabetes was induced by intravenously injecting streptozotocin 55mgkg in 0. Animals were sacrificed six 6n, 6d or nineteen 19n, 19d weeks after diabetes induction. Streptozotocininduced experimental diabetes in male. This protocol is used by diacomp members to induce diabetes in a number of the animal models developed by the consortium. Therapeutic development to prevent bone loss andor restore lost bone in t1 diabetic patients requires knowledge of the molecular mechanisms accounting for the bone pathology. Autoxidation of dialuric acid generates superoxide radicals. Mccabe abstract as many as 50% of adults with type i t1 diabetes exhibit bone loss and are at increased risk for fractures. Induction of diabetes mellitus in rats using intraperitoneal streptozotocin. Stz is toxic to the insulinproducing beta cells of the pancreas and used to induce a diabetes similar to a type i diabetic reference. Streptozotocin stz induced diabetes mellitus dm offers a very costeffective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible.
As a general rule, there is no need to avoid alcohol because you have diabetes. If you are having difficulty downloading or saving the pdf, please refer to our pdf. The males of various strains are differentially susceptible to developing diabetes. Impact of streptozotocininduced diabetes and insulin. Haematological and blood biochemical values were recorded before and after administration of the drug. Here, we thus combined highfathighfructose diet, with multiple lowdose injections of streptozotocin hf.
Evaluation of antidiabetic activity of ethanolic extract. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. The streptozotocindiabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Diabetes in humans induces chronic complications such as cardiovascular damage, cataracts and retinopathy, nephropathy and polyneuropathy. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. At lower doses, stzinduced diabetes is not stable, since spontaneous recovery occurs. Hyperglycemia enhances the production of reactive oxygen species, leading. Ebscohost serves thousands of libraries with premium essays, articles and other content including induction of diabetes mellitus in rats using intraperitoneal streptozotocin. Effect of streptozotocin diabetes and insulin treatment on. The advantages of nongenetic animal models of diabetes compared with the genetically.
It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. The streptozotocininduced diabetic nude mouse model. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. The mechanisms of alloxan and streptozotocininduced. Full text streptozotocininduced type 1 diabetes in. Carbohydrate metabolizing enzymes activity in streptozotocin. Protective role of opn in the islets osteopontin opn is a secrete osteopontin opn is a secreted acidic phosphoprotein that binds to a cell surface integrinbinding motif and is involved in many inflammatory and immunemodulating disorders. Comparative antidiabetic activity of methanolic extract of operculina turpethum stem and root against healthy and streptozotocin induced diabetic rats shankaraiah pulipaka1, srinivasa reddy challa2, ravindra babu pingili3 1trinity college of pharmaceutical sciences, peddapally, karimnagar, andhra pradesh, india, 505 172. Multiple low dose streptozotocininduced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic. Effect of streptozotocin on glucose levels in albino.
Induction of diabetes by streptozotocin in rats article pdf available in indian journal of clinical biochemistry 22222. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. The mechanism of alloxan and streptozotocin action in b. The timecourse changes in the blood glucose levels during the oral glucose tolerance test 0120 min of all groups were shown in figure 2. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. U department of pharmacology, college of health sciences, usmanu danfodiyo university, sokoto, nigera phone. Multiple low dose streptozotocininduced diabetes in the. A comparison between 2 strains of rats article pdf available in european journal of scientific research 323.